AA31-51 are required for nuclear localization of TRIB1.
Top, schema of the deletions and insertions introduced in FLAG-tagged TRIB1. Bottom, immunofluorescence analysis of HeLa cells transfected for 48 h with the indicated TRIB1 mutants. FLAG tagged TRIB1 expressing cells were fixed, permeabilized and examined by confocal microscopy using an antibody specific for the FLAG epitope and an anti-mouse secondary (Alexa 647 goat anti-mouse, Molecular Probes). Nuclei were counterstained with Hoechst 33342.