(A) Body weights of untreated ( = 5), CD8 T cell–transferred ( = 5), or CD4 T cell–transferred ( = 5) mice were monitored for 6 wk. Percentages of the resultant body weights against preinjection body weights were calculated every week. The means and SDs are indicated. (B) Colon tissues were obtained from control or CD8 T cell–transferred mice after 9 wk. Morphology of the representative colon tissues is shown. Bar, 1 cm. (C) HE staining was performed on sections of the colon tissues. Histological pictures of the representative tissues are shown at two different magnifications. Means and SDs of the colitis score are indicated in the bar graph. Bars: (left) 1 mm; (right) 200 μm. (D) Serum KC and SAA levels of untreated or CD8 T cell–transferred mice were measured by ELISA. The means and SDs are indicated. (E) Cytokine (IFN-γ and IL-17) production by CD8 T cells from pLNs and mLNs of the adoptively transferred mice 1, 4, and 6 wk after cell transfer. IFN-γ–producing cells in mLNs or pLNs, and IL-17–producing cells in mLNs or pLNs are indicated. Means and SDs of the percentages of cytokine producing cells are shown. (F) Colon sections from untreated or CD8 T cell–transferred mice were stained with DAPI, anti-CD8 mAb, and anti-CD11b mAb. Bars, 200 μm.
Taken from "IL-6–dependent spontaneous proliferation is required for the induction of colitogenic IL-17–producing CD8 T cells"
The Journal of Experimental Medicine 2008;205(5):1019-1027.
Published online 12 May 2008