20250218_Lieberetal_Figures4and5

FIGURE 4. Har allows S. sanguinis to form robust biofilms at a higher HOSCN concentration than S. mutans. (A, B) CV staining of 75 µM HOSCN-treated and untreated biofilms grown in DROOL: S. sanguinis and its derivatives (A) and S. mutans (B). CV quantification of S. sanguinis (C), S. sanguinis ∆har::kan+ (D), S. sanguinis ∆yumC::kan+ (E), and S. mutans (F) biofilms. HOSCN was added to treated cultures at t = 0 hr. Asterisks represent statistical significance to the respective 0 µM condition (one way ANOVA with Tukey’s post hoc test, * = p<0.05, ** = p<0.01, *** = p<0.001, **** = p<0.0001).

FIGURE 5. HOSCN does not kill S. sanguinis or S. mutans at up to 500 µM in DROOL. CFU counts of 8 hr biofilms grown in DROOL and treated with 0-500 µM HOSCN. (A) S. sanguinis, (B) S. sanguinis ∆har::kan+, (C) S. sanguinis ∆yumC::kan+ and (D) S. mutans. Lines indicate limit of detection (LOD). HOSCN was added to treated cultures at t = 0 hr. Asterisks represent statistical significance to the respective 0 µM condition by one-way ANOVA with Tukey’s post hoc test of log-transformed data (* = p<0.05, ** = p<0.01).