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qPCR validation of indicated RNAi lines.

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posted on 2021-05-13, 17:32 authored by Jun Xie, Yongchao Han, Tao Wang

UAS-RNAi lines were crossed with GMR-GAL4, and adult eyes of F1 flies were dissected for RNA extraction and subsequent qPCR. Samples were prepared in triplicate. All values were normalized to GMR-GAL4 samples. RpL32 was used as the reference gene for standardization. The p-values were obtained using a Student’s T-tests against the GMR-GAL4 samples.