mor_ssDNA/dsDNA_gene_ssDNA/dsDNA_C.hesperidum..pzfx

Invented in 2008, CUAD biotechnology is among three cost-effective antisense technologies used for insect pest control (RNAi, CUAD, and CRISPR/Cas) based on the formation of duplexes of unmodified nucleic acids (RNAi: guide RNA-mRNA; CUAD: guide DNA-rRNA; CRISPR/Cas: guide RNA-genomic DNA) and action of nucleic acid-guided nucleases (RNAi: Argonaute; CUAD: RNase H; CRISPR/Cas: CRISPR-associated protein). It is amazing that simple CUAD-based ‘genetic zipper’ method is so efficient for IPM.