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gliding motility assay using bovine caseins as a surface passivator

Version 4 2016-02-01, 18:41
Version 3 2016-01-28, 05:56
Version 2 2016-01-27, 13:08
Version 1 2016-01-27, 04:50
dataset
posted on 2016-02-01, 18:41 authored by Andy MaloneyAndy Maloney, Lawrence J. Herskowitz, Steven J. koch
Data are from a gliding motility assay using kinesin-1 and rhodamine labeled microtubules. Videos were taken to investigate the effects of surface passivation on the gliding motility assay. Videos are sped up by a factor of 6 and have been false colored.

File names follow the convention: passivatorType_Y-M-D_roi.avi. Note that passivatorType can be any of the following: [Alpha_casein, Beta_casein, Kappa_casein, Mixed_casein, Whole_casein]. Y-M-D is a date indicator, and roi is a zero padded integer value indicating a "Region Of Interest" within the slide.

If you plan to redo speed measurements.
Use the timestamps.json file to get accurate timestamps between frames when converting the avi to individual frames. Each avi should have 600 individual frames.

Please see the given paper for more information about the data and the experiments.

Funding

The experiments were funded by DTRA grant number “HDTRA-1-09-1-0018” and the INCBN from a NSF Grant “DGE-0549500”.

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