posted on 2014-11-11, 03:30authored byTheodoros Goulas, Anna Cuppari, Raquel Garcia-Castellanos, Scott Snipas, Rudi Glockshuber, Joan L. Arolas, F. Xavier Gomis-Rüth
a
Calculated vector sizes based on the electrophoretic mobility with a Lambda DNA marker as standard.
b
For introducing a Strep-tag at the C-terminus of the target protein use a reverse primer without a stop codon.