Figure 5. Intact STING and MAVS signaling is required for Phase I HSV-1 gene expression.

5A-B. Latently infected neurons were transduced with vectors expressing shSTING or shCTRL during latency.
5A. Relative expression of ICP27 mRNA quantified by RT-qPCR at 0 and 18 hours post-reactivation (Phase I) with LY294002 (20μM).
5B. Relative expression of Sting mRNA quantified by RT-qPCR at 0 and 18 hours post-reactivation (Phase I) with LY294002 (20μM).

5C. Titers of infectious virus at 24 hours post-infection of Wild-type, STING KO, or MAVS KO ARPE-19 cells infected with KOS-SPA or KOS-UL98 at an MOI of 5 PFU/cell.  

5D-E. Latently infected neurons were transduced with vectors expressing shMAVS or shCTRL during latency.
5D. Relative expression of ICP27 mRNA quantified by RT-qPCR at 0 and 18 hours post-reactivation (Phase I) with LY294002 (20μM).
5E. Relative expression of Mavs mRNA quantified by RT-qPCR at 0 and 18 hours post-reactivation (Phase I) with LY294002 (20μM).   

5F. Relative expression of ICP27 mRNA quantified by RT-qPCR at 0 and 18 hours post-reactivation (Phase I) following depletion of both STING and MAVS.