Electronic supplementary materials

Electronic supplementary materials

Table S1. List of best-fit substitution models for the alignments of eight aquatic beetle families used in phylogenetic tree reconstruction (Table S1.xlsx).

Table S2. List of aquatic beetles recorded in this study, their BIN assignment, and the total number of sequences uploaded to the BOLD database (Table S2.xlsx).

Table S3. Mean sequence divergence (p-distance) within species, genera and family (Table S3.xlsx).

Table S4A. List of localities with the occurrence of aquatic beetles in the Tatra Mts recorded in this study (Table S4A,B.xlsx).

Table S4B. List of all recorded species of aquatic beetles and their localities in the Tatra Mts supplemented with data from the literature (for the synopsis, see Čiamporová-Zaťovičová & Čiampor, 2011) (Table S4A,B.xlsx).

Table S5. Extended COI alignment and detailed dataset information (603 bp, 1946 sequences) of aquatic beetles, including 65 of the 68 species recorded in this study and public sequences retrieved from the BOLD database (Table S5.xlsx).

Table S6. Results of species delimitation analyses performed using BIN assignment, ASAP, and mPTP approaches on an extended dataset covering seven families of aquatic beetles (Table S6.xlsx).

Table S7. COI alignment and detailed dataset information of 179 haplotypes of aquatic beetles recorded within the Tatra Mts and three outgroup sequences (Table S7.xlsx).

Table S8. Distribution of 179 haplotypes of 68 aquatic beetles and detailed information on their localities in the Tatra Mts (Table S8.xlsx).

Table S9. COI alignments and detail datasets information of cryptic complexes in Hydroporus melanarius, Hydroglyphus geminus, and Hydraena riparia recorded in the Tatra Mts, supplemented with public sequences retrieved from the BOLD database and outgroup sequences. (Table S9.xlsx).

Table S10. Detailed characteristics of the genetic diversity of selected representatives of aquatic beetles from six families in the Tatra Mts (TM populations) and from territories outside this area (WR populations) (Table S10.xlsx).

Figure S1. Haplotype maps of aquatic beetles from the extended dataset, covering all species recorded in the Tatra Mts, supplemented with public sequences retrieved from the BOLD database (Figure .pdf)

Figure S2. Geographic distribution of selected representatives of aquatic beetles from six families from the territory outside the Tatra Mts (WR populations) obtained from the BOLD database (Figure S2.png).