8 files

Unidirectional P-Body Transport during the Yeast Cell Cycle

posted on 11.06.2014, 02:50 by Cecilia Garmendia-Torres, Alexander Skupin, Sean A. Michael, Pekka Ruusuvuori, Nathan J. Kuwada, Didier Falconnet, Gregory A. Cary, Carl Hansen, Paul A. Wiggins, Aimée M. Dudley

P-bodies belong to a large family of RNA granules that are associated with post-transcriptional gene regulation, conserved from yeast to mammals, and influence biological processes ranging from germ cell development to neuronal plasticity. RNA granules can also transport RNAs to specific locations. Germ granules transport maternal RNAs to the embryo, and neuronal granules transport RNAs long distances to the synaptic dendrites. Here we combine microfluidic-based fluorescent microscopy of single cells and automated image analysis to follow p-body dynamics during cell division in yeast. Our results demonstrate that these highly dynamic granules undergo a unidirectional transport from the mother to the daughter cell during mitosis as well as a constrained “hovering” near the bud site half an hour before the bud is observable. Both behaviors are dependent on the Myo4p/She2p RNA transport machinery. Furthermore, single cell analysis of cell size suggests that PBs play an important role in daughter cell growth under nutrient limiting conditions.