Transcription analysis of CPIJ005623 in the Culex pipiens complex.

A–B. CPIJ005623 transcription in adult female (A) and male (B) Culex quinquefasciatus, Pel (Wolbachia-infected) line relative to the Wolbachia-uninfected PelU, over time (days post pupal eclosion). Similar decreasing expression dynamics was seen in both sexes. C–D. Tissue analysis of CPIJ005623 transcription at 4 d in ovaries (C) and 1 d in testes (D) in C. quinquefasciatus Wolbachia-infected lines Pel and Cxq (30), their Wolbachia-free counterparts PelU and CxqT, and Wolbachia-infected Italy line C. molestus. Upregulation of CPIJ005623 expression is seen in all Wolbachia-infected lines. Average of the mean values of four biological repeats (+/− standard error-SE) are presented. Two-way ANOVA statistical analysis was used to determine effect of Wolbachia infection status (wis) and time on CPIJ005623 expression. Wilcoxon rank-sum test was used to determine differences between Wolbachia infection status in CPIJ005623 expression (C–D): *p<0.05, **p<0.01, ***p<0.001.