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Transcription analysis of CPIJ005623 in the Culex pipiens complex.

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posted on 31.10.2013, 20:22 by Sofia B. Pinto, Kirsty Stainton, Simon Harris, Zakaria Kambris, Elizabeth R. Sutton, Michael B. Bonsall, Julian Parkhill, Steven P. Sinkins

A–B. CPIJ005623 transcription in adult female (A) and male (B) Culex quinquefasciatus, Pel (Wolbachia-infected) line relative to the Wolbachia-uninfected PelU, over time (days post pupal eclosion). Similar decreasing expression dynamics was seen in both sexes. C–D. Tissue analysis of CPIJ005623 transcription at 4 d in ovaries (C) and 1 d in testes (D) in C. quinquefasciatus Wolbachia-infected lines Pel and Cxq (30), their Wolbachia-free counterparts PelU and CxqT, and Wolbachia-infected Italy line C. molestus. Upregulation of CPIJ005623 expression is seen in all Wolbachia-infected lines. Average of the mean values of four biological repeats (+/− standard error-SE) are presented. Two-way ANOVA statistical analysis was used to determine effect of Wolbachia infection status (wis) and time on CPIJ005623 expression. Wilcoxon rank-sum test was used to determine differences between Wolbachia infection status in CPIJ005623 expression (C–D): *p<0.05, **p<0.01, ***p<0.001.

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