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Stopped-flow analysis of GroEL D398A-RW.

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posted on 30.10.2013 by Toshifumi Mizuta, Kasumi Ando, Tatsuya Uemura, Yasushi Kawata, Tomohiro Mizobata

A. Analysis of fluorescence intensity changes upon addition of 1black trace indicates GroEL R231W, and the gray trace indicates D398A-RW. B. As in A, but with the duration of observation set to 100 sec. Gray trace indicates D398A-RW with ATP added (as in A), red trace indicates fluorescence changes upon addition of 1 mM AMP-PNP.