Stopped-flow analysis of GroEL D398A-RW.

A. Analysis of fluorescence intensity changes upon addition of 1black trace indicates GroEL R231W, and the gray trace indicates D398A-RW. B. As in A, but with the duration of observation set to 100 sec. Gray trace indicates D398A-RW with ATP added (as in A), red trace indicates fluorescence changes upon addition of 1 mM AMP-PNP.