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Specific activity of Ae.aegypti recombinant ALDH isoforms to oxidise PBald.

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posted on 2014-07-21, 15:27 authored by Nongkran Lumjuan, Jureeporn Wicheer, Posri Leelapat, Wej Choochote, Pradya Somboon

Recombinant ALDH (5 µg) was incubated with 2 mM PBald in the presence of 3 mM NAD+ in 0.1 M Tris-Cl buffer, pH 7.4 at 37°C for 10 min. PBacid formation was determined by HPLC as described. Three independent assays were performed. The results are shown as the mean ± SE.