Purification of recombinant mAOX1 after expression in E. coli TP1000 cells.
datasetposted on 2013-02-21, 06:07 authored by Silvia Schumann, Mineko Terao, Enrico Garattini, Miguel Saggu, Friedhelm Lendzian, Peter Hildebrandt, Silke Leimkühler
Total protein was quantified with the Bradford assay.b
The activity was measured by monitoring the decrease in absorption at 600 nm in the presence of 500 µM benzaldehyde and 100 µM DCPIP.c
Specific enzyme activity (units/mg) is defined as the oxidation of 1 µM benzaldehyde per min and mg of enzyme under the assay conditions.