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Purification of recombinant mAOX1 after expression in E. coli TP1000 cells.

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posted on 21.02.2013, 06:07 by Silvia Schumann, Mineko Terao, Enrico Garattini, Miguel Saggu, Friedhelm Lendzian, Peter Hildebrandt, Silke Leimkühler
a

Total protein was quantified with the Bradford assay.

b

The activity was measured by monitoring the decrease in absorption at 600 nm in the presence of 500 µM benzaldehyde and 100 µM DCPIP.

c

Specific enzyme activity (units/mg) is defined as the oxidation of 1 µM benzaldehyde per min and mg of enzyme under the assay conditions.

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