Table_2.xls (17.5 kB)

Proteins that were significantly altered by the disruption of MMCAP2_0241 in Mycoplasma mycoides subsp. mycoides as determined by iTRAQ™.

Download (0 kB)
posted on 31.12.2012 by Ayman B. Allam, Mary B. Brown, Leticia Reyes

The gene locus was identified using the Molligen database and the M. mycoides subsp. capri GM12, taxon:436113 genome sequence. A protein may be listed in more than one category if it has multiple functions. Accession numbers refer to GenBank ( The protein sequences are available at GenBank ( and via Genomes on Line ( Protein ratios were generated by dividing the spectral intensity of a specific protein in the M. mycoides subsp. capri ctpA (clpP)::tetM mutant by the spectral intensity of the specific protein in M. mycoides capri GM12. Protein ratios were calculated with the Pro Group™ algorithm (Applied Biosystems/MDS SCIEX). Only ratios from the spectra that are distinct to each protein (or protein form) were used for the calculation. Only proteins with 95% or greater confidence and an error factor (EF)<2 were considered. Proteins were decreased in the mutant if the mean ratio was <1.0; proteins were increased in the mutant if the mean ratio was >1.0. Proteins were grouped according to global biologic function as assigned in the Molligen 2.0 database [28] and/or UniProt [29], [30]. Protein ratios were considered significantly different if they had P values <0.01 as determined by the Pro Group™ algorithm [27].