posted on 2013-02-20, 17:38authored byAnna Martinez, El Moukhtar Aliouat, Annie Standaert-Vitse, Elisabeth Werkmeister, Muriel Pottier, Claire Pinçon, Eduardo Dei-Cas, Cécile-Marie Aliouat-Denis
Whole organism population was isolated from the lungs of immunosuppressed animals as previously described [27]. Sporocyte and mature cyst stages were counted from RAL-555-stained smears according to the number of nuclei in each life cycle stage. Percentages of sporocyte and cystic stages with N nuclei in the fraction of sporocytes and mature cysts were given as mean of three independent experiments (n = 70, n = 55, n = 50). N = number of nuclei per fungal cell. SD = standard deviation.
(a)
Early sporocytes.
(b)
Sum of intermediate sporocytes with 2, 3 and 4 nuclei.
(c)
Sum of late sporocytes and mature cysts with 5, 6, 7 and 8 nuclei.