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Production of O2- in thylakoid membranes isolated from control and ALA-treated WT Arabidopsis plants.

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posted on 2013-02-20, 12:31 authored by Gopal K. Pattanayak, Baishnab C. Tripathy

Thylakoid membranes were isolated from leaves before the day-break in dark and were suspended at a concentration of 1 mg Chl ml−1. The thylakoid membranes were illuminated by incandescent light (500 µmoles photons m−2 s−1) passed through heat and neutral density filters. O2- generation by the thylakoid membranes was monitored as cytochrome C reduction.