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Primers used in this study.

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posted on 2013-02-19, 16:52 authored by Jae Myung Lee, Junhyeong Lee, Taesung Kim, Sung Kuk Lee

In envZ deletion and cI insertion, gene specific regions are underlined and P1 and P4 regions are indicated in bold letters.


In pJM1 and pJM2 construction, GAATTC, EcoRI site; GTCGAC, SalI site; AGCTACTAAAGCGTAGTTTTCGTCGTTTGCTGC, LVA site; AATAACACCGTGCGTGTT, CI binding site 1; TTTACCTCTGGCGGTG, CI binding site 2; AAGGAG, strong RBS (0.3); GGATTA, weak RBS (0.1). Homologous regions of primer 7 and primer 8 are indicated in bold letters.