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Primers for specific amplification of Seg-2 of BTV-6, by RT-PCR.

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posted on 2010-04-23, 01:11 authored by Sushila Maan, Narender S. Maan, Piet A. van Rijn, René G. P. van Gennip, Anna Sanders, Isabel M. Wright, Carrie Batten, Bernd Hoffmann, Michael Eschbaumer, Chris A. L. Oura, Abraham C. Potgieter, Kyriaki Nomikou, Peter P.C. Mertens

*Individual primers are identified by the BTV serotype (e.g. BTV-6) followed by the number 2 (to indicate Seg-2), then a number to indicate the relative amino acid position of the primer within VP2, followed by F or R to indicate forward or reverse orientation.

**This primer pair also amplifies Seg-2 of BTV-14 and BTV-21, the most closely related serotypes to BTV-6 within nucleotype ‘C’ and it is therefore regarded as nucleotype ‘C’ specific.