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Primer extension assay in the presence or absence of ATP.

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posted on 13.01.2011 by Atsuko Hachiya, Eiichi N. Kodama, Matthew M. Schuckmann, Karen A. Kirby, Eleftherios Michailidis, Yasuko Sakagami, Shinichi Oka, Kamalendra Singh, Stefan G. Sarafianos
a.

The sequence of HIV RT WT and mutant derived from BH10.

b.

Data are means ± standard deviations from at least three independent experiments.

c.

The relative increase in IC50 value compared with each HIV-1 RT WT without, or with ATP is given in parentheses. Bold indicates an increase in fold increase value greater than 3-fold.

d.

Excision enhancement due to ATP is calculated as IC50 with ATP/IC50 without ATP.

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