Table_1.xls (9.5 kB)

Primer Sequences and Cycling Conditions.

dataset

posted on 2014-01-20, 03:07 authored by Pakieli H. Kaufusi, James F. Kelley, Richard Yanagihara, Vivek R. Nerurkar

a

Construct name. The numbers in parentheses indicate the NS4B amino acid (aa) from 1–255 and the minus sign represent the number of aa to C-teminal ends of NS4A preceding NS4B protein.

b

Primer name. The CT or NT in the primer name denotes C- or N-terminal end of NS4B with respect to GFP fusion protein whereas the F or R denotes the sense or antisense primer, respectively.

c

The Kozak sequence is in bold, lowercase and italicize. The nucleotides in lower case only are codon filling nucleotides. The viral sequences are in uppercase.

*

GenBank Accession No. AF196835.