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Phenotype and HLA class I allele restriction of HSV-1 reactive T-cells recovered from human TG.

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posted on 2013-08-15, 01:55 authored by Monique van Velzen, Lichen Jing, Albert D. M. E. Osterhaus, Alessandro Sette, David M. Koelle, Georges M. G. M. Verjans
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TG-derived T-cell lines were incubated with mock– and HSV-1–infected autologous B-cell lines and assayed by flow cytometry for intra-cellular interferon gamma (IFN-γ) expression.

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The ratio of CD4 and CD8 T-cells of the respective TG-derived T-cell lines are indicated.

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Patient HLA class I allele restricted HSV-1 reactive CD8 T-cell responses were defined using partially HLA class I matched BLCL. The values represent mean net percentages of live/CD3-gated IFN-γ+ T-cells (HSV-1 minus mock) of at least 2 separate experiments.

nd, not done.

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