Kd and Bmax of [3H]-PK 11195 binding using homogenates prepared from cytokine-treated DBT cells.
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posted on 2009-12-18, 00:31 authored by Michelle Sexton, Grace Woodruff, Eiron Cudaback, Faith R. Kreitzer, Cong Xu, Yi Hsing Lin, Thomas Möller, Mingfeng Bai, H. Charles Manning, Darryl Bornhop, Nephi StellaDBT cells incubated for 18–24 hrs with either vehicle (basal), TNFα (5 ng/ml), IFNγ (100 Ui/ml), TNFα+IFNγ, or TGFβ2 (1 ng/ml). Cells were harvested, homogenates prepared and [3H]-PK 11195 Kd and Bmax determined as outlined in the materials and methods section. Values are mean±SEM of 12–15 measurements (i.e. four or five independent experiments, each performed in duplicate). We found no significant difference in Kd and Bmax between basal and respective cytokine-treated conditions, as determined by ANOVA followed by Dunnett's.
