Cotton seedlings were pre-treated with chilling stress for 3 days, then inoculated with A. alternata isolate A1, and removed to grow at optimal temperature of 28/20°C; Different letters behind the disease index indicate significant difference with the P value of 0.05. The first letters indicate the comparison among different temperatures of chilling stress pre-treatment and the second letters indicate the comparison between XLZ13 and XLZ33.