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Inactivation of origins firing in response to UVA-induced ROS in Chk1-deficient MRC5Vi cells.

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posted on 20.10.2015, 04:10 by Dany Graindorge, Sylvain Martineau, Christelle Machon, Philippe Arnoux, Jérôme Guitton, Stefania Francesconi, Céline Frochot, Evelyne Sage, Pierre-Marie Girard

Asynchronous MRC5Vi cells were transfected with siCtr or siChk1 and exposed to 160 kJ/m2 of UVA in MEMi 48h post transfection. IdU and CldU were then sequentially added to the medium 4 h post UVA. The forks density is defined as the ratio between the number of CldU-IdU-CldU signals (red-green-red signals) and the length of replicated DNA. The percentage of cells in S-phase at the labeling time was determined by flow cytometry and was found to be 47%. The forks velocity was recorded only using the CldU tracks length. The data correspond to one experiment.

Inactivation of origins firing in response to UVA-induced ROS in Chk1-deficient MRC5Vi cells.

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