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IFN-γ-based comparison of ELISA, LINCO 29-plex, Bio-Rad 17-plex and RnD Systems Fluorokine-MAP-13-plex assays.

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posted on 2013-02-21, 10:07 authored by Joel Fleury Djoba Siawaya, Teri Roberts, Chantal Babb, Gillian Black, Hawa Jande Golakai, Kim Stanley, Nchinya Bennedict Bapela, Eileen Hoal, Shreemanta Parida, Paul van Helden, Gerhard Walzl

NA: Not applicable because the sample was not measured with the RnD Systems Fluorokine-MAP base kit A

Supernatant was generated using whole blood from a healthy donor (A-E) in six day assays stimulated with 5 differents Mtb derived antigens and with phytohaemagglutinin (PHA). The LINCO 29-plex measurement most closely resembled that of the RnD Systems ELISA in absolute value, and followed a similar trend, but absolute values for all three luminex assay fell below that of the ELISA.

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