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FRET Distances, R0.

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posted on 18.07.2011, 01:58 by Jennifer A. Rochira, Nicholas N. Matluk, Tamara L. Adams, Aldona A. Karaczyn, Leif Oxburgh, Samuel T. Hess, Joseph M. Verdi

Spence MTZ, ed. The Handbook A guide to Fluorescent Probes and Labeling Technologies: Invitrogen Corporation, 2005.

Erickson MG, Moon DL, Yue DT. DsRed as a Potential FRET Partner with CFP and GFP. Biophysical Journal 2003; 85:599–611.

Patterson GH, Piston DW, Barisas BG. Förster Distances between Green Fluorescent Protein Pairs. Analytical Biochemistry 2000; 284:438–40.


Discrepancies between published and calculated FRET distances are possibly due to the specific variability of the experimental environments, choice of published donor quantum yields and acceptor extinction coefficients, and source of fluorescent spectra intensities.