Evaluation of cell death.
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Plasma-treated and H2O2 exposed cells were cultured for 1, 6, 12, 24, and 48 hrs. Cells were then stained with Annexin-V (A) and propidium iodide (PI) and evaluated by FACS analysis to assess cell death in 10,000 events. Data are reported as mean±SE of results collected in nine independent experiments, each performed in duplicate or triplicate.
* denotes P<0.05 vs not treated cells.