Table_3.xls (13.5 kB)

Differentially alternatively spliced candidate genes selected from the Whole Exon Gene Array and RNA-Seq data for RT-PCR validation in activated T cells between 0 and 48 hours.

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posted on 19.11.2009, 01:55 by Yevgeniy A. Grigoryev, Sunil M. Kurian, Aleksey A. Nakorchevskiy, John P. Burke, Daniel Campbell, Steve R. Head, Jun Deng, Aaron B. Kantor, John R. Yates III, Daniel R. Salomon
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ANOVA p-value for Probe Set expression as calculated by XRAY.

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ANOVA p-value for alternative Probe Set usage (alternative splicing) as calculated by XRAY.

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Exons in which the F (forward) and R (reverse) PCR primers are located.

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Known Alternative Splicing Events for the detected Probe Set shown in the UCSC Genome browser.

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Change in alternative splicing as calculated by XRAY analysis at 48 vs 0 hours (activation at 48 hours).

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Change in exon counts (number of reads that align to the exon) as observed from the RNA-Seq data at T48 vs T0 hours. Abbreviations: Ex  =  exon, Int  =  Intron, Ext  =  extended exon, alt-C-term  =  alternative C terminal, alt-3′  =  alternate 3

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