Comparative densitometric analysis of pH and calcium-dependent Yops secretion in <i>Y. pseudotuberculosis</i>.

<p>To compare and quantify the Yop secretion efficiency in wild-type <i>Y. pseudotuberculosis</i> under different conditions, Coomassie stained Yop secretion profiles were subjected to densitometric analysis with Multi Gauge software (Fuji Film). The protein YopJ (boxed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0049349#pone-0049349-g004" target="_blank">Figure 4</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0049349#pone-0049349-g005" target="_blank">Figure 5E</a>) was selected for quantitative analysis. Growth kinetics in media with different pH’s (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0049349#pone.0049349.s004" target="_blank">Figure S4B</a>) showed attenuation of bacterial growth directly linked to the observed Yop secretion efficiency.</p>a<p>secretion efficiency was set to 100%;</p>b<p>secretion efficiency at pH 7.5 was set to 100%.</p>