Co-recruitment of LncRNA-HIT and p100 is required to stimulate gene expression from a synthetic locus.
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(A) Analysis of UAS-luciferase reporter activation after GAL4-λN-BoxBLncRNA-HIT and GAL4-p100 recruitment. (B) Analysis of UAS-luciferase reporter activation in the absence of GAL4-p100. (C) Model of UAS-luciferase reporter activation in response to the recruitment of LncRNA-HIT and p100. For panels A and B, increasing dosages (0, 50, 100, 250, ng) of the BoxB or BoxB-LncRNA-HIT transcripts are represented by the black triangle.