Brassica napus FLC homologues, their map positions, and sequence identities compared with their orthologues in B. rapa or B. oleracea.
The sequences isolated from BACs are indicated by an asterisk, and those isolated from PCR-amplified genomic DNA are designated ‘gDNA’ with the parent indicated in parentheses (T, B. napus cv. Tapidor; N, B. napus cv. Ningyou7).b
The obtained sequences were compared with that of AtFLC to define the regions of each BnFLC gene that were isolated.c
To calculate sequence identities, indels (insertions or deletions) were excluded and for partial sequences of BnFLC.C2 and BnFLC.C3a, the available region was used.d
The letter and numeral in parentheses represent the linkage group followed by the ancestral block in which the FLC homologues are located.e
The number of nonsynonymous nucleotide substitutions is shown in parentheses.f
There was a fragment with higher-order structure in intron 1 of BnFLC.A3a for which we failed to obtain the sequence.