Bioluminescence Amplitude Data for Responses of WT and Vipr2^−/− Tissues to Control and GRP Treatments.

NOTE: Values are presented as mean amplitude change from pre- to post-treatment ± SEM. All treatments were delivered to tissue collected from mice housed under a 12 h∶12 h light∶dark cycle. WT = mPer2^luc-expressing wild type mice; Vipr2^−/− = mPer2^luc-expressing mice lacking functional expression of the VPAC₂ receptor gene.

* = significant difference to control treatment at p<0.05;

** = significant difference to control treatment at p<0.01;

*** = significant difference to control treatment at p<0.005;

**** = significant difference to control treatment at p<0.0005;

 = GRP-treated WT pituitary responses were not significantly different to control responses (as some control-treated WT pituitaries showed an increase in rhythm amplitude), however, GRP did induce a significant increase (p<0.05) in post-treatment vs. pre-treatment amplitude.

Negative values arise due to the normal reduction in rhythm amplitude over time (e.g. see Figs. 2 and 4) when treatments do not induce an increase in amplitude. Numbers in brackets indicate ‘n’ contributing to that data value.