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Binding of Adenocarcinoma aptamers with other cancer cell lines.

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posted on 2012-10-17, 01:58 authored by Elizabeth Jiménez, Kwame Sefah, Dalia López-Colón, Dimitri Van Simaeys, Hui William Chen, Melvyn S. Tockman, Weihong Tan

A threshold based on the fluorescence intensity of FITC in the flow cytometric analysis was chosen so that 99% of cells incubated with the FITC-labeled unselected DNA library would have lower fluorescence intensity than the threshold. When the FITC-labeled aptamer was allowed to interact with the cells, the percentage of the cells with fluorescence above the set threshold was used to evaluate the binding capacity of the aptamer to the cells. (−) 0<10%, 10–35% +, 35–60% ++, 60–85% +++, >85% ++++.

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