figshare
Browse
IMAGE
Figure_S1.tif (3.6 MB)
IMAGE
Figure_S2.tif (3.23 MB)
IMAGE
Figure_S3.tif (7.18 MB)
IMAGE
Figure_S4.tif (5.73 MB)
IMAGE
Figure_S5.tif (5.73 MB)
IMAGE
Figure_S6.tif (5.73 MB)
IMAGE
Table_S1.tif (11.46 MB)
1/0
7 files

Amino Acid Starvation Has Opposite Effects on Mitochondrial and Cytosolic Protein Synthesis

dataset
posted on 2014-04-09, 03:36 authored by Mark A. Johnson, Sara Vidoni, Romina Durigon, Sarah F. Pearce, Joanna Rorbach, Jiuya He, Gloria Brea-Calvo, Michal Minczuk, Aurelio Reyes, Ian J. Holt, Antonella Spinazzola

Amino acids are essential for cell growth and proliferation for they can serve as precursors of protein synthesis, be remodelled for nucleotide and fat biosynthesis, or be burnt as fuel. Mitochondria are energy producing organelles that additionally play a central role in amino acid homeostasis. One might expect mitochondrial metabolism to be geared towards the production and preservation of amino acids when cells are deprived of an exogenous supply. On the contrary, we find that human cells respond to amino acid starvation by upregulating the amino acid-consuming processes of respiration, protein synthesis, and amino acid catabolism in the mitochondria. The increased utilization of these nutrients in the organelle is not driven primarily by energy demand, as it occurs when glucose is plentiful. Instead it is proposed that the changes in the mitochondrial metabolism complement the repression of cytosolic protein synthesis to restrict cell growth and proliferation when amino acids are limiting. Therefore, stimulating mitochondrial function might offer a means of inhibiting nutrient-demanding anabolism that drives cellular proliferation.

History