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ATHB17Δ113 can bind both Class II and Class I DNA targets and ATHB17Δ113 containing V182A-Q185A-N186A mutation cannot bind Class II DNA target in in vitro assay (measured by Surface Plasmon Resonance (SPR).

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posted on 15.04.2014, 04:30 by Elena A. Rice, Abha Khandelwal, Robert A. Creelman, Cara Griffith, Jeffrey E. Ahrens, J. Philip Taylor, Lesley R. Murphy, Siva Manjunath, Rebecca L. Thompson, Matthew J. Lingard, Stephanie L. Back, Huachun Larue, Bonnie R. Brayton, Amanda J. Burek, Shiv Tiwari, Luc Adam, James A. Morrell, Rico A. Caldo, Qing Huai, Jean-Louis K. Kouadio, Rosemarie Kuehn, Anagha M. Sant, William J. Wingbermuehle, Rodrigo Sala, Matt Foster, Josh D. Kinser, Radha Mohanty, Dongming Jiang, Todd E. Ziegler, Mingya G. Huang, Saritha V. Kuriakose, Kyle Skottke, Peter P. Repetti, T. Lynne Reuber, Thomas G. Ruff, Marie E. Petracek, Paul J. Loida

Binding affinities and Kinetic constants of ATHB17Δ113 interacting with Class I and Class II type DNA, measured by Biacore 2000, globally fitted. SPR measurements with Biacore 2000 were at 25°C in HBS-EP, 100 ug/ml BSA (10 mM HEPES pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.005% Tween-20,100 ug/ml BSA). Equilibrium dissociation constant KD = koff/kon.

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