We assembled a chromosome-scale genome of wild Chinese chestnut, and a total of 2,652,199 read pairs (90.55 Gb) were acquired by Hi-C sequencing, offering over 115-fold coverage. Then, to improve gene prediction accuracy, high-quality transcripts of 11 mixed tissue types were sequenced using a single-molecule real-time sequencer from Pacific Biosciences. Finally, a total of 33,991 protein-coding genes were aligned to the wild Chinese chestnut genome (V2).
Funding
the National Key Research & Development Program of China (2018YFD1000605)
the National Natural Science Foundationof China (31870671)