WT and NitroTyr Calmodulin Mass Spectrometry Data

Purified CaM samples were diluted to a concentration of 10 μM, desalted on Millipore C4 zip tips, and analyzed using an FT LTQ mass spectrometer. Samples included WT-CaM and nitroTyr-containing CaM. Spectra were collected using Tune-Plus (Thermo, v. 2.2) page of Xcalibur (Thermo, v. 2.0.5) using parameters described in (Rhoads, T. W., Williams, J. R., Lopez, N. I., Morre, J. T., Bradford, C. S., and Beckman, J. S. (2013) Using theoretical protein isotopic distributions to parse small-mass-difference post-translational modifications via mass spectrometry. J Am Soc Mass Spectrom 24, 115-124). Spectra were averaged over the three minutes CaM eluted from the ZipTip using the Qual Browser (Thermo, v. 2.0) The data were exported as text files containing two columns of m/z versus intensity to be analyzed by custom programs written in MatLab (Rhoads et al., 2013). This data includes the raw m/z and deconvoluted data in tabs for the WT-, nitroTyr99-, and nitroTyr138-CaM.