Underlying data for the experiments of Figs 3 and S1.

In addition to the underlying β-galactosidase measurements for Fig 3C and quantitation of the Western blots for Fig 3D, the file contains the following information. One tab in the file shows auto-activation controls for the four-hybrid experiments of Fig 3C, showing no significant binding of AD-Nos in yeast expressing (1) various NLS-Puf domain fusions and the RPR1 vector-encoded RNA only (i.e., bearing neither NRE nor FBE) (2) protein encoded by the empty NLS-fusion vector (i.e., neither Pum nor FBF2) and either the NRE- or FBE-containing hybrid RNAs. Another tab shows no substantial difference between the level of RNA-binding activity of Pum[NTR + RBD] and its F1367S mutant derivative in three-hybrid experiments. Additional, tabs contain the source data for S1B-S1D Fig.

(XLSX)