Transcriptome and Metabolite Conjoint Analysis Reveals that Exogenous Methyl Jasmonate Regulates Monoterpene Synthesis in Grape Berry Skin
datasetposted on 2020-04-27, 14:11 authored by Wei Li, Wenfang Li, Shijin Yang, Zonghuan Ma, Qi Zhou, Juan Mao, Shunyu Han, Baihong Chen
Monoterpene is one of the important sources of varietal aroma, which provides a strong floral and fruity aroma in wines. Methyl jasmonate (MeJA) affects plant secondary metabolism. However, the regulatory mechanisms of monoterpene biosynthesis after MeJA application on grapes are not illuminated. In the present study, 10 mM MeJA was used as treatments in Italian Riesling grape at the preveraison stage in different ways, including grape cluster soaking, foliar spraying, and whole vine spraying, designated as T1, T2, and T3, respectively, while a blank group was used as the control (CK). HS-SPME/GC-MS and transcriptome sequencing analysis were performed to investigate the effect of exogenous MeJA on monoterpene synthesis in grape berry skin. The results of GC-MS showed that the application of MeJA induced the accumulation of volatile monoterpenes in grape berry skin, especially linalool, α-terpineol, and oxides. In addition, transcriptome analysis showed that differentially expressed genes were increased from T2 to T3 to T1 compared with CK, and significantly enriched in JA and monoterpene synthesis pathways. T1 application significantly upregulated the mRNA expression levels of LOX2S, AOS, OPR, and JMT involved in the JA biosynthesis pathway, as well as DXS, HMGCR, TPS14, and α-terpineol synthesis genes involved in the monoterpene synthesis pathway compared with T2, T3, and CK. Thus, grape cluster soaking treatment with MeJA could greatly activate volatile monoterpene synthesis. The results will deeply increase our understanding of the monoterpene biosynthesis of grape berry skin in response to MeJA.
HMGCRAOSα- terpineol synthesis genes2Smonoterpene biosynthesismonoterpene synthesis pathwaysmRNA expression levelsGrape Berry Skin MonoterpeneJMT10 mM MeJAgrape clusterGC-MSOPRgrape berry skinCKmonoterpene synthesisMetabolite Conjoint AnalysisT 1 applicationLOXmonoterpene synthesis pathwayExogenous Methyl Jasmonate Regulates Monoterpene SynthesisItalian Riesling grapeJA biosynthesis pathwayDXSHS-SPMETPStranscriptome sequencing analysis