The Arctic moss Aulacomnium turgidum and the Antarctic moss Polytrichastrum alpinum play crucial roles in the ecosystems of polar regions, contributing significantly to biodiversity and ecological stability. We sequenced and de novo assembled the genomes of A. turgidum and P. alpinum using a combination of Illumina short reads, Nanopore long reads, and Hi-C scaffolding technology. The genome sizes are 277.84 Mb for A. turgidum and 498.33 Mb for P. alpinum, anchored to 11 chromosomes and 8 chromosomes, respectively. Both species exhibit a sex chromosome with distinct genomic characteristics. The genomes encode 25,999 protein-coding genes in A. turgidum and 28,070 in P. alpinum.