Table_1_CD11c+ B Cells Are Mainly Memory Cells, Precursors of Antibody Secreting Cells in Healthy Donors.XLS
CD11c+ B cells have been reported to be increased in autoimmune diseases, but they are detected in the blood of healthy individuals as well. We aimed to characterize CD11c+ B cells from healthy donors by flow cytometry, microarray analysis, and in vitro functional assays. Here, we report that CD11c+ B cells are a distinct subpopulation of B cells, enriched in the memory subpopulation even if their phenotype is heterogeneous, with overexpression of genes involved in B-cell activation and differentiation as well as in antigen presentation. Upon activation, CD11c+ B cells can differentiate into antibody-secreting cells, and CD11c could be upregulated in CD11c− B cells by B-cell receptor activation. Finally, we show that patients with pemphigus, an autoimmune disease mediated by B cells, have a decreased frequency of CD11c+ B cell after treatment, relative to baseline. Our findings show that CD11c+ B cells are mainly memory B cells prone to differentiate into antibody secreting cells that accumulate with age, independently of gender.
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References
- https://doi.org//10.3389/fimmu.2017.01866
- https://doi.org//10.1007/978-3-642-74977-3_6
- https://doi.org//10.1002/eji.1830260304
- https://doi.org//10.1182/blood-2006-04-014878
- https://doi.org//10.1084/jem.181.4.1473
- https://doi.org//10.4049/jimmunol.164.11.5928
- https://doi.org//10.1073/pnas.88.3.1044
- https://doi.org//10.1189/jlb.0612273
- https://doi.org//10.1182/blood-2011-01-331462
- https://doi.org//10.1002/art.37828
- https://doi.org//10.4049/jimmunol.1502448
- https://doi.org//10.1038/s41467-018-03750-7
- https://doi.org//10.4049/jimmunol.0901297
- https://doi.org//10.4049/jimmunol.1303214
- https://doi.org//10.4049/jimmunol.1501027
- https://doi.org//10.1002/eji.201847641
- https://doi.org//10.1016/j.cll.2007.05.001
- https://doi.org//10.1038/s41598-019-40203-7.
- https://doi.org//10.3389/fimmu.2019.01794
- https://doi.org//10.1186/gb-2002-3-7-research0034
- https://doi.org//10.1038/nprot.2008.211
- https://doi.org//10.1128/MCB.01155-06
- https://doi.org//10.1074/jbc.M707692200
- https://doi.org//10.1016/j.cyto.2015.02.007
- https://doi.org//10.1016/j.immuni.2019.04.004
- https://doi.org//10.1016/j.coi.2008.03.003
- https://doi.org//10.3389/fimmu.2017.01938
- https://doi.org//10.1073/pnas.0911193107
- https://doi.org//10.1016/S0140-6736(17)30070-3
- https://doi.org//10.1084/jem.20050879
- https://doi.org//10.1186/s12967-018-1404-z
- https://doi.org//10.4049/jimmunol.1801236
- https://doi.org//10.1016/j.immuni.2018.08.015
- https://doi.org//10.1101/723585
- https://doi.org//10.1084/jem.20072683
- https://doi.org//10.1172/jci.insight.92943
- https://doi.org//10.1371/journal.ppat.1004894
- https://doi.org//10.4049/jimmunol.176.1.191
- https://doi.org//10.4049/jimmunol.1401917
- https://doi.org//10.1007/s12026-012-8365-8
- https://doi.org//10.1074/jbc.M101216200
- https://doi.org//10.1073/pnas.1005599107
- https://doi.org//10.1038/s41590-018-0056-8
- https://doi.org//10.1016/S1074-7613(02)00324-2
- https://doi.org//10.4049/jimmunol.173.4.2479
- https://doi.org//10.1016/j.jaut.2014.04.001
- https://doi.org//10.4049/jimmunol.1500368
- https://doi.org//10.1182/blood-2011-01-330530
- https://doi.org//10.1182/blood-2009-09-243071
- https://doi.org//10.1172/JCI91250
- https://doi.org//10.1073/pnas.1312348110
- https://doi.org//10.1084/jem.20081242
- https://doi.org//10.1016/S1074-7613(04)00076-7
- https://doi.org//10.1038/ni941
- https://doi.org//10.1073/pnas.082114899
- https://doi.org//10.4049/jimmunol.1500055
- https://doi.org//10.1016/j.jid.2017.05.032
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Categories
- Transplantation Immunology
- Tumour Immunology
- Immunology not elsewhere classified
- Immunology
- Veterinary Immunology
- Animal Immunology
- Genetic Immunology
- Applied Immunology (incl. Antibody Engineering, Xenotransplantation and T-cell Therapies)
- Autoimmunity
- Cellular Immunology
- Humoural Immunology and Immunochemistry
- Immunogenetics (incl. Genetic Immunology)
- Innate Immunity