Supplementary Table3 Glycine soja and Glycine max metadata.csv

Currently, domesticated species available on the market have been developed through the breeding of wild relatives. We collected gene expression data of domesticated species of rice, tomato, and soybean, along with their presumed ancestral species, and conducted a meta-analysis to investigate differentially expressed genes during domestication. In wild relatives, the expression of gene groups involved in osmotic, drought, and wound stress tolerance was upregulated. In contrast, in domesticated species, upregulated ex-pression was observed in gene groups related to auxin and those involved in the efflux of heavy metals and harmful substances. By identifying these genes with differential ex-pression levels, we can gain insights into how domestication has influenced changes in crop traits. In particular, breeding strategies that make use of wild relatives with high genetic diversity are attracting attention as an important approach for addressing climate change and ensuring a sustainable food supply. The findings obtained in this study are expected to contribute to the realization of rapid breeding and the development of new varieties capable of growing in harsh natural environments. To address this issue, a new cultivation method called "De novo domestication" has been proposed, which combines the genetic diversity of currently unused wild relatives and wild relatives with genome editing technologies that enable rapid breeding.