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Supplementary Material A; Observation Records.xlsx

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posted on 2025-03-13, 16:35 authored by Trefor SimmonsTrefor Simmons, Greg HurstGreg Hurst, Pol Nadal-Jimenez

1. Number of Columns/Variables: 39


2. Number of Rows/Cases: 288


3. Variable List:


A. Name:Treatment Type [Column A]

Description: The method of introduction of A.apicola into the G.mellonella model was either [Injected] through the last left abdominal proleg or [Force-fed].

B. Name: Dose [Column B]

Description:The approximate dose administered to each larvae in colony forming units, estimated by serial dilution plate counts.

C. Name: Individual [Column C]

Description: The individual larva number within the entire cohort, included for ease of reference to specific individuals.

D. Name: Survival Status [Columns D-L]

Description: Larvae were scored each day for survival: 0= alive, 1= dead, c= censored. Larvae in the [Injected] treatment group were only monitored for 7 days so were considered to be censored on the 8th day, unlike the [Force-fed] treatment group.


E. Name: Infection Status [Columns M-U]

Description: Larvae were scored each day for infection: 0= uninfected, 1= infected, c= censored. Larvae in the [Injected] treatment group were only monitored for 7 days so were considered to be censored on the 8th day, unlike the [Force-fed] treatment group.

F. Name: Melanisation Status [Columns V-AD]

Description: Larvae were scored each day for melanistaion: 0= unmelanised, 1= melanised, c= censored. Larvae in the [Injected] treatment group were only monitored for 7 days so were considered to be censored on the 8th day, unlike the [Force-fed] treatment group. Melanised status allocated where melanisation was scored at 2 or more according to scoring criteria of Kay et al 2019 Frontiers in Microbiology 10: 1281..


G. Name: Pupation Status [Columns AE-AM]

Description: Larvae were scored each day for pupation: 0= unpupated, 1= pupated, c= censored. Larvae in the [Injected] treatment group were only monitored for 7 days so were considered to be censored on the 8th day, unlike the [Force-fed] treatment group.


Funding

BBSRC grant BB/S017534/1

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