Sulfur-cycling diazotrophs dominate nitrogen fixation in seagrass sediments

<p dir="ltr">If you use this data, please acknowledge.</p><p dir="ltr">Clean metagenomic reads were aligned to their assembly using Bowtie2 v2.4.4 (default parameters) (Langmead and Salzberg, 2012) to determine contig coverage. The assembled sequences were binned using MetaBAT2 v2.12.1 (Kang et al., 2019) and CONCOCT v1.1.0 (Alneberg et al., 2014), applying a contig length cut-off of 1.5 kb. The resulting bin sets were refined with the metaWRAP Bin_refinement module (parameters: -c 50) (Uritskiy et al., 2018). All generated bins were combined and dereplicated to create a non-redundant set of MAGs using dRep v3.2.2 (parameters: -comp 50 -con 10 --P_ani 0.9 --S_ani 0.98) (Olm et al., 2017) at 98% average nucleotide identities.</p><p dir="ltr">The identification of diazotroph MAGs was conducted using the Diaiden pipeline (<a href="https://github.com/jchenek/Diaiden" target="_blank">https://github.com/jchenek/Diaiden</a>).</p>