Single nuclei gene expression matrix from 5 regions of the human prenatal brain

Data generated through single nuclei RNA sequencing on 5 regions of the brain (frontal cortex, ganglionic eminence, hippocampus, thalamus and cerebellum) from 3 fetuses (two of 14 and one of 15 post-conception weeks, all female). Tissue was acquired from the MRC-Wellcome Trust Human Developmental Biology Resource (HDBR) with ethical approval.  

snRNA-seq libraries were prepared from ∼10,000 nuclei from each sample using Chromium Single Cell 3ʹ (v3) reagents (10X Genomics). Quality control of libraries was performed using the Agilent 5200 Fragment Analyzer before sequencing on an Illumina NovaSeq 6000 to a depth of at least 865 million (median = 1.01 billion) read pairs per library. Raw sequencing data were converted into FASTQ files, aligned to the hg38 build of the human reference genome and quantified using cellranger count (10X Genomics).       

For a full description of data generation, please see Cameron et al, Biological Psychiatry 2022                           https://doi.org/10.1016/j.biopsych.2022.06.033