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Real-time deformability cytometry data of primary human skeletal stem cells and the human osteosarcoma cell line MG-63

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posted on 20.01.2020, 12:02 by Miguel Xavier, Philipp Rosendahl, Paul MüllerPaul Müller, Maik Herbig, Jochen Guck
Real-time deformability data of primary human skeletal stem cells and the human osteosarcoma cell line MG-63

The MG-63 cell line is used as a model system for human skeletal stem cells (SSC). See references [1] and [2] for more information. The file names follow the pattern "[sample]_[flow rate/reservoir]_[repetition]_[date].rtdc", i.e. the file "SSC_32uls_rep1_20150611.rtdc" contains human skeletal stem cell data measured at a flow rate of 32µl/s - it is the first of two repetitions.


This is a subset of the data used by Xavier et al. [1] to quantify the stiffness of MG-63 and SSC cells. Herbig et al. [2] used the 16µl/s data in section IIID to illustrate linear mixed model analysis.


References
[1] M. Xavier et al., Mechanical phenotyping of primary human skeletal stem cells in heterogeneous populations by real-time deformability cytometry, Integrative Biology, 2016, doi:10.1039/C5IB00304K

[2] M. Herbig et al., Statistics for real-time deformability cytometry: Clustering, dimensionality reduction, and significance testing, Biomicrofluidics, 2018. doi:10.1063/1.5027197

Funding

Alexander von Humboldt Foundation

Sächsisches Ministerium für Wissenschaft und Kunst (TG70)

European Union’s Seventh Framework Programme under Grant Agreement No. 632222

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