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Real-time deformability cytometry reference data

dataset
posted on 14.09.2019, 13:36 by Philipp Rosendahl, Christoph Herold, Paul MüllerPaul Müller, Jochen Guck
This dataset consists of four exemplary real-time fluorescence and deformability cytometry measurements. The HDF5-files can be opened with dclab [1] or Shape-Out [2].

calibration_beads.rtdc
The calibartion beads (8 Peaks, PolyAN) consist of eight bead populations with different mixtures of fluorophores.


CD34_HSPC.rtdc
Hematopoietic stem and progenitor cells (HSPCs) were obtained using apheresis. The cells were tagged with a fluorescently labeled antibody that binds to the CD34 transmembrane protein. CD34-positive HSPCs are gated with `fl3_max > 90`. Set `area_ratio < 1.05` to remove aggregates. Data were used in [3].


leukocytes.rtdc
The leukocyte population (white blood cells) of this blood sample can be visualized by setting `aspect < 2` and `area_ratio < 1.05`. For more information, see e.g. [4].


reticulocytes.rtdc
Blood contains mostly red blood cells (RBCs) and about 1% reticulocytes (which develop into mature RBCs). Reticulocytes contain ribosomal RNA which was stained with Syto13 for this measurement. Set `area_ratio < 1.05` to remove aggregates. Data were used in [3].


[1] https://github.com/ZellMechanik-Dresden/dclab

[2] https://github.com/ZellMechanik-Dresden/ShapeOut

[3] Rosendahl et al., "Real-time fluorescence and deformability cytometry". Nature Methods, 15(5):355–358, 2018. doi:10.1038/nmeth.4639.

[4] Toepfner et al., "Detection of human disease conditions by single-cell morpho-rheological phenotyping of whole blood". eLife, 7:e29213, 2017. doi:10.1101/145078.


SHA256 sums:
08c2ef13eed903ef0f9e451727ab8484df09b5d3b39227dab726e0164dcbe244 calibration_beads.rtdc
663b44a9db88d85996500045489e37a317cf115719223a531d617f8e3d450e79 CD34_HSPC.rtdc
68bd538b42ffb990f1db52d5f3b21f37c9aff31208ab284f3910fd6872c40fdb leukocytes.rtdc
5c323ea75bf7eeb2a28d922730772d50270dd872d6957e60d6062663f3628fb3 reticulocytes.rtdc

Funding

Alexander-von-Humboldt Stiftung (Humboldt-Professorship to J.G.)

Sächsisches Ministerium für Wissenschaft und Kunst (TG70)

European Fund for Regional Development

ERC Starting Grant ("LightTouch" #282060)

DFG Center for Regenerative Medicine of the Technische Universität Dresden

DFG KFO249 (GU 612/2-2)

History