Raw Data_ZC 20241105.xlsx

We investigated Polyphosphate (polyP) cycling in a cross-latitudinal section (15°N-39°N, 150°E) of the Northwest Pacific Ocean, covering a diverse nutrient gradient and varying P stress conditions, 2023. This data set includes biogeochemical parameters as follows, polyP, Chl a, nutrients, alkaline phosphatase activity (APA), picophytoplankton, total particulate phosphorus (TPP). Detailed methods can be as follows,

We used a conductivity-temperature-depth (CTD) instrument cluster (Seabird 911) to collect seawater samplers.

Seawater samples were filtered onto GF/F filters to obtain polyP and TPP samplers. PolyP was quantified using a fluorescence method according to the protocol described by Martin et al., 2013, doi: 10.1128/AEM.02592-12. TPP was converted into phosphate and quantified using a UV spectrophotometer (Shi et al., 2019, doi: 10.5194/acp-19-847-2019).

APA was calculated through the change of fluorescence using a Turner Trilogy fluorometer, details can be seen in Jin et al., 2024, doi: 10.1038/s41467-024-44838-7.

Nutrient samplers were filtered through syringe filters (0.45 μm in core size) and determined by a QuAAtro Continuous-Flow Analyzer (SEAL Analytical) (Grasshoff et al., 1999, Methods of seawater analysis).

Chl a samplers were filtered onto Whatman GF/F filters (47 mm in diameter) and were determined using a Turner Trilogy fluorometer (Strickland & Parsons, 1972, A Practical Handbook of Seawater Analysis).

Picophytoplankton was determined using a Beckman CytoFLEX Flow Cytometer (Wei et al., 2020, doi: 10.3354/ame01928).