Rainbow trout (Oncorhynchus mykiss) skin digital images

160 rainbow trout were fed either a commercial based diet (80 fish) or a 100 % plant-based diet (80 fish) and photographed using consumer-grade digital camera. Each live fish was photographed with a 12-megapixel Nikon D3300 digital camera (Nikon Corp., Tokyo, Japan) under a lighting system consisting of four halogen lamps (200 W bulb) at an angle of 45 degrees, and 35 cm above the sample to not only provide constant intensity output but also to give the uniform light intensity over the fish sample. Images were collected in a dark room with only light source, coming through halogen lamps cast on fish skin. Digital camera was located vertically at 56 cm from the sample. All setting on the camera were on manual. The setting of camera was: exposure mode = manual, shutter speed = 1/160 s, aperture = f/4.0, ISO sensitivity = 100. The images were recorded in Nikon Raw format (NEF) and transferred to a laptop for further processing.